NFkB p65 (phospho Ser529) antibody | GeneTex GTX50254 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

NFkB p65 (phospho Ser529) antibody

catalog :

GTX50254

quantity :

100.00μl

price :

299 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

product information

Catalog No :

GTX50254

Product Name :

NFkB p65 (phospho Ser529) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Product Type :

Primary antibody

Size :

100.00μl

List Price :

299 USD

Product Description :

Rabbit Polyclonal antibody to NF kappa B-p65 (Phospho Ser529)

CrossReactivity :

Hu, Ms, Rat

Background :

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

Immunogen :

Peptide sequence around phosphorylation site of serine 529 (L-L-S(p)-G-D) derived from Human NF kappa B-p65.

Buffer :

saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Uses :

ICC/IF, IHC, IHC-P, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Western blot analysis of extracts from 293 cells untreated or treated with TNF-a using NFkB-p65(Phospho-Ser529) Antibody GTX50254

Image Legend_5 :

WB analysis of of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB p65 (phospho Ser529) antibody.