HIF1 alpha antibody | GeneTex GTX30647 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

HIF1 alpha antibody

catalog :

GTX30647

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

Catalog No :

GTX30647

Product Name :

HIF1 alpha antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

21129412,26136949

Product Description :

Rabbit polyclonal antibody to mouse HIF 1 alpha

CrossReactivity :

Hu, Ms, Rat, Goat, Hm, NHuPrm, Rb

Background :

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.

Immunogen :

A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.

Specificity :

This antibody is specific for mouse HIF-1 alpha.

Buffer :

0.1 ml of protein affinity purified antisera containing 0.05% sodium azide.

Uses :

ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, ChIP assay

Concentration :

1mg/ml

General References :

Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.

Image Legend_4 :

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in human kidney tumor

Image Legend_5 :

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in paraffin section of mouse skin wound using GTX30647 at 1:6,000.

Image Legend_6 :

Western Blot: HIF-1 alpha Antibody [GTX30647] - Detection of HIF-1 alpha in a hypoxic sample Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.

Image Legend_7 :

Western Blot: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.