HIF2 alpha antibody | GeneTex GTX30114 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

HIF2 alpha antibody

catalog :

GTX30114

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

Catalog No :

GTX30114

Product Name :

HIF2 alpha antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

11565642,10878807,11734856,11793372,25010988,25796140

Isotype :

IgG

Product Description :

Rabbit polyclonal to HIF - 2 alpha (EPAS1)

CrossReactivity :

Hu, Ms, Rat, Fsh

Background :

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)

Immunogen :

A peptide derived from the mouse/human HIF-2 alpha.

Specificity :

Specific for HIF-2 alpha/EPAS. Does not cross react with HIF-1 alpha.

Buffer :

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.

Image Legend_5 :

Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114

Image Legend_6 :

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500.

Image Legend_7 :

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000.

Image Legend_8 :

HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: U-87 MG cells CoCl2 treated with 200μM CoCl2 for 24 hrs, and add 10μM MG132 for 3 hrs before fixation and were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody (GTX30114) diluted at 1:1000. Blue: Hoechst 33342 staining.