HIF1 alpha antibody [H1alpha67] | GeneTex GTX30105 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

HIF1 alpha antibody [H1alpha67]

catalog :

GTX30105

quantity :

100.00μl

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H1alpha67

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 3

Reference
Kuo H, Lee D, Xia W, Wei Y, Hung M. TNFalpha induces HIF-1alpha expression through activation of IKKbeta. Biochem Biophys Res Commun. 2009;389:640-4 pubmed publisher
Catrina S, Botusan I, Rantanen A, Catrina A, Pyakurel P, Savu O, et al. Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Clin Cancer Res. 2006;12:4506-14 pubmed
Nonomura Y, Mizoguchi F, Suzuki A, Nanki T, Kato H, Miyasaka N, et al. Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation. J Rheumatol. 2009;36:698-705 pubmed publisher

product information

Catalog No :

GTX30105

Product Name :

HIF1 alpha antibody [H1alpha67]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μl

List Price :

299 USD

list of Pubmed id :

16899596,19766100,19228663

Clone :

H1alpha67

Isotype :

IgG2b

Product Description :

Mouse monoclonal [H1alpha67] to Hypoxia Inducible Factor-1 alpha

CrossReactivity :

Hu, Ms, Pig, Rat, Yeast, Frt, NHuPrm, Rb, Shp, Bov

Background :

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.

Immunogen :

Amino acids 432-528 of human HIF-1alpha.

Specificity :

This antibody is specific for HIF-1 alpha.

Buffer :

Phosphate-buffered saline containing 0.02% sodium azide

Uses :

ELISA, FACS, GSA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB, ChIP assay

General References :

Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.

Image Legend_4 :

MEF cells were grown on 15 cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs.

Image Legend_5 :

HIF-1 alpha Antibody [H1alpha67] (GTX30105) - Detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.

Image Legend_6 :

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-HIF-1 alpha antibody [H1alpha67] (GTX30105).

Image Legend_7 :

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) (GTX30105) - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Image Legend_8 :

Western Blot: HIF-1 alpha Antibody (H1alpha67) (GTX30105) - Analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts