AKT (phospho Ser473) antibody | GeneTex GTX28932 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

AKT (phospho Ser473) antibody

catalog :

GTX28932

quantity :

100.00μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:150; loading ...; fig 5a western blot; mouse; 1:1500; loading ...; fig 4b,4c	Wang X, Chen H, Tian R, Zhang Y, Drutskaya M, Wang C, et al. Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nat Commun. 2017;8:14091 pubmed publisher

product information

Catalog No :

GTX28932

Product Name :

AKT (phospho Ser473) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Product Type :

Primary antibody

Size :

100.00μg

list of Pubmed id :

22842013,22951000

Isotype :

IgG

Product Description :

Rabbit polyclonal to AKT (phospho S473)

CrossReactivity :

Hu, Ms

Background :

Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways (reviewed in ref 1). Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation (reviewed in 2). Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Immunogen :

Synthetic peptide: CHFPQFS p YSAS conjugated to KLH, corresponding to amino acids 460-480 of Human AKT.

Specificity :

Akt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.

Buffer :

Phosphate-buffered saline, pH 7.3, 50% Glycerol and 150mM NaCl containing 0.02% sodium azide

Uses :

ICC/IF, IHC-P, WB

Concentration :

1mg/ml

General References :

Store vial at -20 °C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Image Legend_4 :

Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT by immunohistochemistry in human breast tumor tissue. The staining is much stronger than the weak basal level of phosphorylation in normal breast.Rabbit anti-AKT pS473 antibody was used with no pretreatment of tissue.Tissue was formalin-fixed and paraffin embedded.

Image Legend_5 :

This image is a magnification of the accompanying breast tumor image.

Image Legend_6 :

Rabbit anti-AKT pS473 was used at a 1:200 dilution to detect phosphorylated AKT by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.

Image Legend_7 :

Immunofluorescence Confocal Microscopy of Rabbit anti-AKT pS473 antibody (GTX28932). Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.