GAPDH antibody [6C5] | GeneTex GTX28245 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

GAPDH antibody [6C5]

catalog :

GTX28245

quantity :

100.00μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6C5

reactivity :

human, domestic rabbit

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

GeneTex product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1	Dong H, Zou M, Bhatia A, Jayaprakash P, Hofman F, YING Q, et al. Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90Î±) to Survive a Hostile Hypoxic Environment. Sci Rep. 2016;6:20605 pubmed publisher
western blot; human	Jayaprakash P, Dong H, Zou M, Bhatia A, O Brien K, Chen M, et al. Hsp90Î± and Hsp90Î² together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing. J Cell Sci. 2015;128:1475-80 pubmed publisher
	Solis G, Kazemzadeh A, Abrami L, Valnohova J, Alvarez C, van der Goot F, et al. Local and substrate-specific S-palmitoylation determines subcellular localization of Gαo. Nat Commun. 2022;13:2072 pubmed publisher
	de Wispelaere M, Du G, Donovan K, Zhang T, Eleuteri N, Yuan J, et al. Small molecule degraders of the hepatitis C virus protease reduce susceptibility to resistance mutations. Nat Commun. 2019;10:3468 pubmed publisher
	Vaquié A, Sauvain A, Duman M, Nocera G, Egger B, Meyenhofer F, et al. Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration. Cell Rep. 2019;27:3152-3166.e7 pubmed publisher
	Guo J, Jayaprakash P, Dan J, Wise P, Jang G, Liang C, et al. PRAS40 Connects Microenvironmental Stress Signaling to Exosome-Mediated Secretion. Mol Cell Biol. 2017;37: pubmed publisher
	Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, et al. Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Nat Commun. 2017;8:14272 pubmed publisher
	Salzman D, Nakamura K, Nallur S, Dookwah M, Metheetrairut C, Slack F, et al. miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nat Commun. 2016;7:10954 pubmed publisher
	Meng J, Lee S, Hotard A, Moore M. Refining the balance of attenuation and immunogenicity of respiratory syncytial virus by targeted codon deoptimization of virulence genes. MBio. 2014;5:e01704-14 pubmed publisher
	Fuerst E, Foster H, Ward J, Corrigan C, Cousins D, Woszczek G. Sphingosine-1-phosphate induces pro-remodelling response in airway smooth muscle cells. Allergy. 2014;69:1531-9 pubmed publisher
	Kiryu M, Niwano S, Niwano H, Kishihara J, Aoyama Y, Fukaya H, et al. Angiotensin II-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model. Europace. 2012;14:1206-14 pubmed publisher
	Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini L, et al. Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells. J Cell Mol Med. 2012;16:2186-95 pubmed publisher
	Hsu S, Chen T, Chou Y, Chen L, Kuo C, Lee T, et al. Extra-nuclear activation of progesterone receptor in regulating arterial smooth muscle cell migration. Atherosclerosis. 2011;217:83-9 pubmed publisher

product information

Catalog No :

GTX28245

Product Name :

GAPDH antibody [6C5]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

list of Pubmed id :

21440892,22454409,25041788,25249281

Clone :

6C5

Isotype :

IgG1

Product Description :

Mouse monoclonal [6C5] to GAPDH

CrossReactivity :

Hu, Ms, Dog, Pig, Rat, Cat, Fsh, Rb

Background :

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Immunogen :

Rabbit muscle GAPDH.

Specificity :

This antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast.

Buffer :

Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide

Uses :

Apuri, CC, Control, ELISA, ICC/IF, IP, Puri, WB

Concentration :

12.8mg/ml

General References :

Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer.

more info or order :

GeneTex product webpage