CD34 antibody [MEC14.7] | GeneTex GTX28158 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

CD34 antibody [MEC14.7]

catalog :

GTX28158

quantity :

100.00μg

price :

289 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MEC14.7

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 10

Reference
Urbani L, Piccoli M, Franzin C, Pozzobon M, De Coppi P. Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential. PLoS ONE. 2012;7:e49860 pubmed publisher
Trempus C, Morris R, Bortner C, Cotsarelis G, Faircloth R, Reece J, et al. Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. J Invest Dermatol. 2003;120:501-11 pubmed
Tchaicha J, Reyes S, Shin J, Hossain M, Lang F, McCarty J. Glioblastoma angiogenesis and tumor cell invasiveness are differentially regulated by β8 integrin. Cancer Res. 2011;71:6371-81 pubmed publisher
Spirli C, Okolicsanyi S, Fiorotto R, Fabris L, Cadamuro M, Lecchi S, et al. ERK1/2-dependent vascular endothelial growth factor signaling sustains cyst growth in polycystin-2 defective mice. Gastroenterology. 2010;138:360-371.e7 pubmed publisher
Tchaicha J, Mobley A, Hossain M, Aldape K, McCarty J. A mosaic mouse model of astrocytoma identifies alphavbeta8 integrin as a negative regulator of tumor angiogenesis. Oncogene. 2010;29:4460-72 pubmed publisher
Uchibori R, Tsukahara T, Mizuguchi H, Saga Y, Urabe M, Mizukami H, et al. NF-κB activity regulates mesenchymal stem cell accumulation at tumor sites. Cancer Res. 2013;73:364-72 pubmed publisher
Truman J, Garcia-Barros M, Kaag M, Hambardzumyan D, Stancevic B, Chan M, et al. Endothelial membrane remodeling is obligate for anti-angiogenic radiosensitization during tumor radiosurgery. PLoS ONE. 2010;5:e12310 pubmed publisher
Hirahashi J, Hishikawa K, Kaname S, Tsuboi N, Wang Y, Simon D, et al. Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury. Circulation. 2009;120:1255-65 pubmed publisher
Schem C, Bauerschlag D, Bender S, Lorenzen A, Loermann D, Hamann S, et al. Preclinical evaluation of sunitinib as a single agent in the prophylactic setting in a mouse model of bone metastases. BMC Cancer. 2013;13:32 pubmed publisher
Peng Y, Pan C, Hsieh Y, Wu S, Chu H, Hsu W. The application of tissue engineering in reversing mitomycin C-induced ischemic conjunctiva. J Biomed Mater Res A. 2012;100:1126-35 pubmed publisher

product information

Catalog No :

GTX28158

Product Name :

CD34 antibody [MEC14.7]

Host Species :

Rat

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

List Price :

289 USD

list of Pubmed id :

23166781,12648211,20531304,20808818,22337602,21859829,19766642,23066037,19752320,23347638

Clone :

MEC14.7

Isotype :

IgG2a

Product Description :

Rat monoclonal [MEC14.7] to CD34

CrossReactivity :

Hu, Ms

Background :

The antibody is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore the antibody can be used for isolation and characterization of hematopoietic progenitor cells. In summary the antibody can be used for ELISA, immunofluorescence, immunohistochemistry on frozen and paraffin sections, Western blot, immunoprecipitation, flow cytometry and cell separation. Western blot: The reported molecular mass of CD34 is ranging from 100 to 120 kDa, Apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).

Specificity :

Reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. Furthermore the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.

Buffer :

Phosphate-buffered saline containing 0.02% sodium azide

Uses :

ELISA, FACS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.