ERK1/2 (phospho Thr185/Tyr187) antibody | GeneTex GTX24819 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

ERK1/2 (phospho Thr185/Tyr187) antibody

catalog :

GTX24819

quantity :

50.00μl

price :

289 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, rat

application :

western blot

citations: 3

Reference
Tong T, Fan W, Zhao H, Jin S, Fan F, Blanck P, et al. Involvement of the MAP kinase pathways in induction of GADD45 following UV radiation. Exp Cell Res. 2001;269:64-72 pubmed
Robinson F, Whitehurst A, Raman M, Cobb M. Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1. J Biol Chem. 2002;277:14844-52 pubmed
Janulis M, Trakul N, Greene G, Schaefer E, Lee J, Rosner M. A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity. Mol Cell Biol. 2001;21:2235-47 pubmed

product information

Catalog No :

GTX24819

Product Name :

ERK1/2 (phospho Thr185/Tyr187) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Product Type :

Primary antibody

Size :

50.00μl

List Price :

289 USD

list of Pubmed id :

11823456,11238956,11525640

Isotype :

IgG

Product Description :

Rabbit polyclonal antibody to ERK1/2 (phospho Thr185/Tyr187)

CrossReactivity :

Background :

ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signalling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK 1 and ERK 2 are fully activated by phosphorylation within a Thr-Glu-Tyr motif of the activation loop on both a threonine and a tyrosine residue by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of the ERK 1 + 2 enzymes.

Immunogen :

Synthetic peptide (Human) derived from the region of ERK 1 + 2 that contains threonine 202/185 and tyrosine 204/187, based on the human sequence. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.

Specificity :

The sequence of the immunogenic peptide used to generate GTX24819 includes the amino acids found in the region of both ERK1 and ERK2 including specific threonine and tyrosine residues. In ERK1, these residues are at positions 185 and 187, respectively. In ERK2, these same residues are actually at positions 202 and 204, respectively of the native protein. The antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.

Buffer :

Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3

Uses :

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.