Fibrillarin antibody [38F3] | GeneTex GTX24566 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

Fibrillarin antibody [38F3]

catalog :

GTX24566

quantity :

250.00μl

price :

289 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

38F3

The same clone is also sold as:

BioLegend: 905001
Invitrogen: MA1-22000, MA3-16771, 480009
Rockland Immunochemicals: 900-301-D39
OriGene: TA336627
MyBioSource: MBS500003
Novus Biologicals: NB300-269
LifeSpan Biosciences: LS-C2552, LS-C204517

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

GeneTex product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:200; loading ...; fig 2a	Behm M, Wahlstedt H, Widmark A, Eriksson M, Ohman M. Accumulation of nuclear ADAR2 regulates A-to-I RNA editing during neuronal development. J Cell Sci. 2017;: pubmed publisher
immunocytochemistry; mouse; 1 ng/ml; fig 12	Mitrea D, Cika J, Guy C, Ban D, Banerjee P, Stanley C, et al. Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA. elife. 2016;5: pubmed publisher
	Zougman A, Mann M, Wisniewski J. Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene. Nucleic Acids Res. 2011;39:7103-13 pubmed publisher

image

image 1 :

Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000

image 2 :

Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue).

image 3 :

High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixat

product information

Catalog No :

GTX24566

Product Name :

Fibrillarin antibody [38F3]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

250.00μl

List Price :

289 USD

list of Pubmed id :

21586586

Clone :

38F3

Isotype :

IgG1

Product Description :

Mouse monoclonal [38F3] to Fibrillarin - Nucleolar Marker

CrossReactivity :

Hu, Ms, Dm, Rat, Ce, S. pombe, plnt

Background :

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Immunogen :

Yeast nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).

Buffer :

Preservative: 10mM Sodium Azide; Constituents: Tissue Culture Supernatant

Uses :

ICC/IF, IHC, IHC-P, WB

Concentration :

tissue culture supernatant

General References :

Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Image Legend_4 :

Blot of yeast protein extract stained with the GTX24566 anti-Gibrillarin anitbody, stained a single band at ~34kDa.

more info or order :

GeneTex product webpage