P Glycoprotein antibody [C219] | GeneTex GTX23364 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

P Glycoprotein antibody [C219]

catalog :

GTX23364

quantity :

250.00μl

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C219

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

GeneTex product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; fig s2 western blot; mouse; 1:2000; fig s5 immunocytochemistry; human; fig 1 western blot; human; 1:2000; fig 1	Kim D, Bynoe M. A2A adenosine receptor modulates drug efflux transporter P-glycoprotein at the blood-brain barrier. J Clin Invest. 2016;126:1717-33 pubmed publisher
western blot; human; fig 4	Deng L, Li Q, Lin G, Huang D, Zeng X, Wang X, et al. P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3. Theranostics. 2016;6:204-18 pubmed publisher
western blot; human; loading ...; fig 1a	FantappiÃ¨ O, Sassoli C, Tani A, Nosi D, Marchetti S, Formigli L, et al. Mitochondria of a human multidrug-resistant hepatocellular carcinoma cell line constitutively express inducible nitric oxide synthase in the inner membrane. J Cell Mol Med. 2015;19:1410-7 pubmed publisher
	Machado S, Cunha V, Reis Henriques M, Ferreira M. Histopathological lesions, P-glycoprotein and PCNA expression in zebrafish (Danio rerio) liver after a single exposure to diethylnitrosamine. Environ Toxicol Pharmacol. 2014;38:720-32 pubmed publisher
	Hu K, Li H, Ou R, Li C, Yang X. Tissue accumulation and toxicity of isothiazolinone in Ctenopharyngodon idellus (grass carp): association with P-glycoprotein expression and location within tissues. Environ Toxicol Pharmacol. 2014;37:529-35 pubmed publisher
	Yokoi K, Tanei T, Godin B, van de Ven A, Hanibuchi M, Matsunoki A, et al. Serum biomarkers for personalization of nanotherapeutics-based therapy in different tumor and organ microenvironments. Cancer Lett. 2014;345:48-55 pubmed publisher
	Komori Y, Arisawa S, Takai M, Yokoyama K, Honda M, Hayashi K, et al. Ursodeoxycholic acid inhibits overexpression of P-glycoprotein induced by doxorubicin in HepG2 cells. Eur J Pharmacol. 2014;724:161-7 pubmed publisher
	Lee Y, Lin T, Chang C, Lo Y. Galectin-3 silencing inhibits epirubicin-induced ATP binding cassette transporters and activates the mitochondrial apoptosis pathway via ?-catenin/GSK-3? modulation in colorectal carcinoma. PLoS ONE. 2013;8:e82478 pubmed publisher
	Gong J, Luk F, Jaiswal R, George A, Grau G, Bebawy M. Microparticle drug sequestration provides a parallel pathway in the acquisition of cancer drug resistance. Eur J Pharmacol. 2013;721:116-25 pubmed publisher
	Yamasaki T, Fujinaga M, Kawamura K, Hatori A, Yui J, Nengaki N, et al. Evaluation of the P-glycoprotein- and breast cancer resistance protein-mediated brain penetration of 11C-labeled topotecan using small-animal positron emission tomography. Nucl Med Biol. 2011;38:707-14 pubmed publisher
	Pelis R, Shahidullah M, Ghosh S, Coca Prados M, Wright S, Delamere N. Localization of multidrug resistance-associated protein 2 in the nonpigmented ciliary epithelium of the eye. J Pharmacol Exp Ther. 2009;329:479-85 pubmed publisher

product information

Catalog No :

GTX23364

Product Name :

P Glycoprotein antibody [C219]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

250.00μl

List Price :

299 USD

list of Pubmed id :

24370495,24370567,24561531,21718946,19201990,24095666,24303084,25299848,25691007

Clone :

C219

Isotype :

IgG2a

Product Description :

Mouse monoclonal [C219] to P Glycoprotein

CrossReactivity :

Hu, Ms, Rat, Zfsh, Hm

Background :

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Immunogen :

SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.

Specificity :

Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein. Specific reactivity to human, mouse, rat, dog and primate. Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot.

Buffer :

Phosphate-buffered saline containing 1% BSA and <0.1% sodium azide

Uses :

FACS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

Concentration :

Batch dependent

General References :

Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

more info or order :

GeneTex product webpage