Myosin Light chain (phospho Ser20) antibody | GeneTex GTX22480 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Myosin Light chain (phospho Ser20) antibody

catalog :

GTX22480

quantity :

50.00μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig 7g	Smith R, Huang Y, Tian T, Vojtasova D, Mesalles Naranjo O, Pollard S, et al. The Transcription Factor Foxg1 Promotes Optic Fissure Closure in the Mouse by Suppressing Wnt8b in the Nasal Optic Stalk. J Neurosci. 2017;37:7975-7993 pubmed publisher

product information

Catalog No :

GTX22480

Product Name :

Myosin Light chain (phospho Ser20) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Product Type :

Primary antibody

Size :

50.00μg

list of Pubmed id :

19820184,22031541,22021442

Isotype :

IgG

Product Description :

Rabbit polyclonal to Myosin light chain ( phospho S20 )

CrossReactivity :

Hu, Ms

Background :

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Immunogen :

Human Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27).

Specificity :

This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2

Uses :

ELISA, ICC/IF, IHC-P, WB

Concentration :

0.75mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.