Collagen I antibody | GeneTex GTX20292 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

Collagen I antibody

catalog :

GTX20292

quantity :

100.00μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; fig 3	Chuang H, Su H, Li C, Lin S, Yen S, Huang M, et al. The Role of Butylidenephthalide in Targeting the Microenvironment Which Contributes to Liver Fibrosis Amelioration. Front Pharmacol. 2016;7:112 pubmed publisher

product information

Catalog No :

GTX20292

Product Name :

Collagen I antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μg

Isotype :

IgG

Product Description :

Rabbit polyclonal to Collagen I

CrossReactivity :

Hu, Rat

Background :

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Immunogen :

Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

Specificity :

Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. This antibody reacts with most mammalian Type I collagens. Minimum Cross Reactivity to Type II, III, IV, V and VI Collagens.

Buffer :

Preservative: 0.01% Sodium Azide; Constituents: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8.0, sterile filtered

Uses :

ELISA, IHC-Fr, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage add 50% glycerol, aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Image Legend_4 :

Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms

Image Legend_5 :

Western blot analysis is shown using GeneTex Affinity Purified anti-Collagen I antibody (GTX20292) to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml. Proteins were detected by a chemiluminescent method.