Caspase 3 antibody [31A1067] | GeneTex GTX13585 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

Caspase 3 antibody [31A1067]

catalog :

GTX13585

quantity :

100.00μg

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

31A1067

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

product information

Catalog No :

GTX13585

Product Name :

Caspase 3 antibody [31A1067]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

List Price :

299 USD

Clone :

31A1067

Isotype :

IgG1

Product Description :

Mouse monoclonal antibody [31A1067] to Caspase 3

CrossReactivity :

Background :

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Immunogen :

Recombinant fusion protein (6xHis tagged full-length human Caspase 3 protein expressed in bacteria).

Buffer :

Preservative: 0.02% Sodium Azide; Constituents: PBS

Uses :

IHC-P, WB

Concentration :

0.5mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Image Legend_4 :

Formalin-fixed, paraffin-embedded normal colon stained with Caspase-3 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis. Cancer/normal adjacent tissue array was used for this test.