antibody

Calnexin antibody

GTX13504

100.00μl

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat, bovine

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

Calnexin antibody

Rabbit

Polyclonal

Primary antibody

100.00μl

IgG

Rabbit polyclonal to Calnexin - ER Marker

Hu, Ms, Cow, Mk, Pig, Rat, Gpig, Hm, Ql, Rb, Shp, Xl

Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

Synthetic peptide: EEDEILNRSPRNRKPRRE conjugated to KLH, corresponding to amino acids 575-593 of Human Calnexin.

Preservative: NoneConstituents: Whole Serum

FACS, ICC/IF, IHC, IP, WB

1mg/ml

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.