ATG9A antibody | GeneTex GTX128427 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

ATG9A antibody

catalog :

GTX128427

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; fig 4	Karanasios E, Walker S, Okkenhaug H, Manifava M, Hummel E, Zimmermann H, et al. Autophagy initiation by ULK complex assembly on ER tubulovesicular regions marked by ATG9 vesicles. Nat Commun. 2016;7:12420 pubmed publisher
western blot; mouse; loading ...; fig s5i	Liu Y, Takahashi Y, Desai N, Zhang J, Serfass J, Shi Y, et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 2016;6:20453 pubmed publisher
	Zhang X, Wang L, Ireland S, Ahat E, Li J, Bekier Ii M, et al. GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion. Autophagy. 2019;:1-14 pubmed publisher
	Merrill N, Schipper J, Karnes J, Kauffman A, Martin K, Mackeigan J. PI3K-C2? knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE. 2017;12:e0184909 pubmed publisher

product information

Catalog No :

GTX128427

Product Name :

ATG9A antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to ATG9A (ATG9 autophagy related 9 homolog A (S. cerevisiae))

CrossReactivity :

Hu, Ms, Rat

Background :

Plays a role in autophagy.

Immunogen :

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9A. The exact sequence is proprietary.

Buffer :

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

ATG9A antibody detects ATG9A protein at on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ATG9A antibody (GTX128427) dilution: 1:500.

Image Legend_5 :

ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 100% MeOH for 5 min. Green: ATG9A protein stained by ATG9A antibody (GTX128427) diluted at 1:500. Blue: Hoechst 33342 staining.

Image Legend_6 :

ATG9A antibody immunoprecipitates ATG9A protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427) 7.5 % SDS-PAGE The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]