LC3B antibody | GeneTex GTX127375 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

LC3B antibody

catalog :

GTX127375

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - frozen section

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:200; loading ...; fig 4a	Viana G, Priestman D, Platt F, Khan S, Tomatsu S, Pshezhetsky A. Brain Pathology in Mucopolysaccharidoses (MPS) Patients with Neurological Forms. J Clin Med. 2020;9: pubmed publisher
western blot; human; loading ...; fig 2a	Tsai C, Li C, Cheng Y, Lee C, Liao P, Lin C, et al. The inhibition of lung cancer cell migration by AhR-regulated autophagy. Sci Rep. 2017;7:41927 pubmed publisher
western blot; human; loading ...; fig 3	Chen K, Lin C, Huang C, Chen S, Wu S, Chiang H, et al. Dual Roles of 17-? Estradiol in Estrogen Receptor-dependent Growth Inhibition in Renal Cell Carcinoma. Cancer Genomics Proteomics. 2016;13:219-30 pubmed
	Chen J, Hsieh Y, Lo H, Li A, Chou C, Yang P. In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells. Biomolecules. 2019;9: pubmed publisher
	Wang J, Wei H, Huang Y, Chen D, Zeng G, Lian Y, et al. The combination of lonafarnib and sorafenib induces cyclin D1 degradation via ATG3-mediated autophagic flux in hepatocellular carcinoma cells. Aging (Albany NY). 2019;11:5769-5785 pubmed publisher
	Wu Y, Lin C, Lin Y, Su W, Chiu W. Autophagy regulates vinorelbine sensitivity due to continued Keap1-mediated ROS generation in lung adenocarcinoma cells. Cell Death Discov. 2018;4:33 pubmed publisher
	Yi H, Wang K, Du B, He L, Ho H, Qiu M, et al. Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells. Molecules. 2018;23: pubmed publisher
	Sullivan P, Zhou X, Robins A, Paushter D, Kim D, Smolka M, et al. The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway. Acta Neuropathol Commun. 2016;4:51 pubmed publisher

product information

Catalog No :

GTX127375

Product Name :

LC3B antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

25227736,25203626,23514754,25686800,23514754

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to LC3B (microtubule-associated protein 1 light chain 3 beta)

CrossReactivity :

Hu, Ms, Pig, Rat

Background :

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

Immunogen :

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.

Specificity :

Cross-reactivity may occur with other LC3 isoforms

Buffer :

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

FACS, ICC/IF, IP, WB

Concentration :

0.83mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

LC3B antibody detects MAP1LC3B protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000

Image Legend_5 :

LC3B antibody detects MAP1LC3B protein by Western blot analysis. A. 50 µg Rat brain lysate/extract 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000

Image Legend_6 :

LC3B antibody detects LC3B protein in Thapsigargin-treated samples by Western blot analysis. Upper panel: LC3B antibody (GTX127375) Lower panel: Beta-actin antibody (GTX110564)A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr) 15 % SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 Beta-actin antibody (GTX110564) dilution: 1:20000

Image Legend_7 :

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Image Legend_8 :

LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000.

Image Legend_9 :

LC3B antibody detects LC3B protein by western blot analysis. Un-treated (-) and treated (+, 50uM Chloroquine treatment for 48 hr) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) at a dilution of 1:500.The beta Actin was used as internal control (GTX10564, 1:50000) shown at the bottom panel.

Image Legend_10 :

Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 μg of LC3B antibody (GTX127375). Western blot analysis was performed using LC3B antibody (GTX127375). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_11 :

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.