Histone H2A.XS139ph (phospho Ser139) antibody | GeneTex GTX127340 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Histone H2A.XS139ph (phospho Ser139) antibody

catalog :

GTX127340

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 2c	Wei R, Lin S, Wu W, Chen L, Li C, Chen H, et al. A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells. Toxicol Appl Pharmacol. 2016;311:88-98 pubmed publisher
immunocytochemistry; human; loading ...; fig 5b western blot; human; loading ...; fig 5a	Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed publisher
	Calo E, Gu B, Bowen M, Aryan F, Zalc A, Liang J, et al. Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders. Nature. 2018;554:112-117 pubmed publisher

product information

Catalog No :

GTX127340

Product Name :

Histone H2A.XS139ph (phospho Ser139) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

25242635

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Histone H2A.X (phospho Ser139) (H2A histone family, member X)

CrossReactivity :

Hu, Ms

Background :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Buffer :

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Sample (30 ug of whole cell lysate) A: HCT116 control 0hr B: HCT116 treated with 30uM cisplatin for 24hr 12% SDS PAGE GTX127340 diluted at 1:1000

Image Legend_5 :

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated) B. 30 µg NIH-3T3 whole cell lysate/extract (30µM cisplatin treatment for 24hr) 15 % SDS-PAGE Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000

Image Legend_6 :

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:250.