Histone H3 antibody | GeneTex GTX122148 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Histone H3 antibody

catalog :

GTX122148

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 4

Reference
Hao H, Hao S, Chen H, Chen Z, Zhang Y, Wang J, et al. N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. Nucleic Acids Res. 2019;47:362-374 pubmed publisher
Lopez Charcas O, Espinosa A, Alfaro A, Herrera Carrillo Z, Ramírez Cordero B, Cortes Reynosa P, et al. The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity. Sci Rep. 2018;8:12995 pubmed publisher
Hollingworth R, Horniblow R, Forrest C, Stewart G, Grand R. Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus. J Virol. 2017;91: pubmed publisher
Yang W, Campbell M, Chang P. SUMO modification of a heterochromatin histone demethylase JMJD2A enables viral gene transactivation and viral replication. PLoS Pathog. 2017;13:e1006216 pubmed publisher

product information

Catalog No :

GTX122148

Product Name :

Histone H3 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

22283874,25227736,25393278,25909282

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Histone H3 (histone cluster 1, H3a)

CrossReactivity :

Hu, Ms, Dm, Mk, Rice

Background :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Immunogen :

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Buffer :

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB, ChIP assay

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Image Legend_5 :

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Image Legend_6 :

Histone H3 antibody detects HIST1H3A protein by western blot analysis. A. 30 μg A431 whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone H3 antibody (GTX122148) dilution: 1:1000