Histone H3K9ac (acetyl Lys9) antibody [AH3-120] | GeneTex GTX12179 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

Histone H3K9ac (acetyl Lys9) antibody [AH3-120]

catalog :

GTX12179

quantity :

50.00μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

acetylated

clone name :

AH3-120

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse	Nair M, Nagamori I, Sun P, Mishra D, Rhéaume C, Li B, et al. Nuclear regulator Pygo2 controls spermiogenesis and histone H3 acetylation. Dev Biol. 2008;320:446-55 pubmed publisher

product information

Catalog No :

GTX12179

Product Name :

Histone H3K9ac (acetyl Lys9) antibody [AH3-120]

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Acetylated

Product Type :

Primary antibody

Size :

50.00μl

list of Pubmed id :

18614164

Clone :

AH3-120

Isotype :

IgG1

Product Description :

Mouse monoclonal [AH3 - 120] to Histone H3 ( acetyl K9 )

CrossReactivity :

Hu, Ms, Dm, Rat

Background :

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Acetylation of lysine residues within these N-terminal domains by histone acetyl-transferases (HATs), including Gcn5p, P/CAF, p300/CBP, and TAFII250, is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. Conversely, histone deacetylation by histone deacetylases (HDACs) is associated with transcription repression reversing the chromatin remodeling process. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.

Immunogen :

A synthetic, acetylated histone H3 peptide (amino acids 7-20, acetyl K9) corresponding to the N-terminus of human histone H3, conjugated to KLH.

Specificity :

Staining of (acetyl K9) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide.

Buffer :

Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4

Uses :

ELISA, ICC/IF, IHC, WB

Concentration :

2.1mg/ml

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.