Histone H3K27me3 (trimethyl Lys27) antibody | GeneTex GTX121184 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Histone H3K27me3 (trimethyl Lys27) antibody

catalog :

GTX121184

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

methylated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

Catalog No :

GTX121184

Product Name :

Histone H3K27me3 (trimethyl Lys27) antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

22549509,25203626

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Histone H3 (tri-Methyl K27) (histone cluster 1, H3a)

CrossReactivity :

Hu, Ms

Background :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Buffer :

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, WB

Concentration :

0.5mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Histone H3 (tri-Methyl K27)(GTX121184) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Image Legend_5 :

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histon H3 (tri-Methyl K27)(GTX121184) antibody at 1:500 dilution.

Image Legend_6 :

Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 7.5% SDS PAGE GTX121184 diluted at 1:500