Bmi1 antibody | GeneTex GTX114008 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

Bmi1 antibody

catalog :

GTX114008

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

product information

Catalog No :

GTX114008

Product Name :

Bmi1 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Bmi1 (BMI1 polycomb ring finger oncogene)

CrossReactivity :

Hu, Ms

Background :

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Immunogen :

Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Bmi1 antibody detects BMI1 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody (GTX114008) dilution: 1:1000

Image Legend_5 :

Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract . B. 30 μg THP-1 whole cell lysate/extract. 10 % SDS-PAGE. Bmi1 antibody (GTX114008) dilution: 1:1000

Image Legend_6 :

Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 μg of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Image Legend_7 :

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bmi1 antibody (GTX114008) dilution: 1:500.

Image Legend_8 :

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bmi1 antibody (GTX114008) dilution: 1:500.