alpha Tubulin antibody [DM1A] (ascites) | GeneTex GTX11302 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

alpha Tubulin antibody [DM1A] (ascites)

catalog :

GTX11302

quantity :

100.00μl

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DM1A

reactivity :

human, mouse, chicken, bovine

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1	Wei P, Lo W, Su M, Shew J, Lee W. Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic Acids Res. 2012;40:323-32 pubmed publisher
	Wei P, Wang Z, Lo W, Su M, Shew J, Chang T, et al. A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress. Nucleic Acids Res. 2013;41:1533-43 pubmed publisher
	Wei P, Hsieh Y, Su M, Jiang X, Hsu P, Lo W, et al. Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Mol Cell. 2012;48:747-59 pubmed publisher
	Wang D, Olman M, Stewart J, Tipps R, Huang P, Sanders P, et al. Downregulation of FIP200 induces apoptosis of glioblastoma cells and microvascular endothelial cells by enhancing Pyk2 activity. PLoS ONE. 2011;6:e19629 pubmed publisher
	Li H, Huang E, Su P, Wu S, Yang C, Lin Y, et al. Nuclear export and import of human hepatitis B virus capsid protein and particles. PLoS Pathog. 2010;6:e1001162 pubmed publisher

product information

Catalog No :

GTX11302

Product Name :

alpha Tubulin antibody [DM1A] (ascites)

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

23241391,21060813,23123197,21908404,21602932

Clone :

DM1A

Isotype :

IgG1

Product Description :

Mouse monoclonal antibody [DM1A] to alpha Tubulin

CrossReactivity :

Hu, Ms, Cow, Amph, Fsh, Fungi, Hm

Background :

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.GT211302 may be used to study the intracellular organization and distribution of tubulin along the static and dynamic aspects of the cytoskeleton. The product can be used for the immunocytochemical localization of the alpha chain of tubulin by means of indirect immunofluorescent labeling of cultured cells, formalin fixed or frozen tissues or for specific identification of the alpha-tubulin band in immunoblotting or immunoprecipitation techniques.

Immunogen :

Purified chick brain tubulin.

Buffer :

Raw Ascites containing 15mM sodium azide

Uses :

ICC/IF, IHC-P, IP, WB, IB

Concentration :

raw ascites

General References :

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Image Legend_4 :

Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A (GTX11302) with Anti-Mouse-Alexa 488 (green) and DAPI (blue).

Image Legend_5 :

Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A (GTX11302).

Image Legend_6 :

Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-α-Tubulin (GTX11302). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)