Calreticulin antibody | GeneTex GTX111627 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Calreticulin antibody

catalog :

GTX111627

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1a	Huang J, Ku W, Chen Y, Chang Y, Chu C. Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Sci Rep. 2017;7:42853 pubmed publisher
immunocytochemistry; human; 1:1000; fig 7 western blot; human; 1:1000; fig 7	Singh N, Johnstone D, Martin K, Tempera I, Kaplan M, Denny M. Alterations in nuclear structure promote lupus autoimmunity in a mouse model. Dis Model Mech. 2016;9:885-97 pubmed publisher
	Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher

product information

Catalog No :

GTX111627

Product Name :

Calreticulin antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

23178681

Product Description :

Rabbit Polyclonal antibody to Calreticulin

CrossReactivity :

Hu, Ms, Rat

Background :

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human Calreticulin. The exact sequence is proprietary.

Buffer :

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Calreticulin antibody detects Calreticulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at a dilution of 1:1000.