HSP60 antibody | GeneTex GTX110089 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

HSP60 antibody

catalog :

GTX110089

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5a	Wakae K, Nishiyama T, Kondo S, Izuka T, Que L, Chen C, et al. Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation. Sci Rep. 2018;8:9745 pubmed publisher
immunocytochemistry; human; 1:500; fig 5 western blot; human; 1:5000; fig 3	Qi Y, Qiu Q, Gu X, Tian Y, Zhang Y. ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts. Sci Rep. 2016;6:24700 pubmed publisher

product information

Catalog No :

GTX110089

Product Name :

HSP60 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

24359494,21742798,22777893,23813585,24727571,25052713,25259860

Product Description :

Rabbit Polyclonal antibody to HSP60 (heat shock 60kDa protein 1 (chaperonin))

CrossReactivity :

Hu, Ms, Rat

Background :

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ELISA, ICC/IF, IHC-P, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX110089 diluted at 1:20000

Image Legend_5 :

HSP60 antibody detects HSP60 protein at mitochondrion on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP60 antibody (GTX110089) dilution: 1:500.

Image Legend_6 :

HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP60 antibody (GTX110089) dilution: 1:500.

Image Legend_7 :

HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP60 antibody (GTX110089) dilution: 1:500.

Image Legend_8 :

HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HSP60 antibody (GTX110089) dilution: 1:500.

Image Legend_9 :

HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP60 protein stained by HSP60 antibody (GTX110089) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.