O-GlcNAc transferase antibody | GeneTex GTX109939 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

O-GlcNAc transferase antibody

catalog :

GTX109939

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:3000; fig 1	Nagai Okatani C, Minamino N. Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure. PLoS ONE. 2016;11:e0150210 pubmed publisher

product information

Catalog No :

GTX109939

Product Name :

O-GlcNAc transferase antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

22345628,22846177

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))

CrossReactivity :

Hu, Ms, Rat

Background :

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.

Buffer :

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.

Image Legend_5 :

Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 μg of O-GlcNAc transferase antibody (GTX109939). Western blot analysis was performed using O-GlcNAc transferase antibody (GTX109939). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_6 :

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

Image Legend_7 :

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.