MAD1 antibody | GeneTex GTX109519 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

MAD1 antibody

catalog :

GTX109519

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:800; fig 5 western blot; human; 1:8000; fig 3	Zhou H, Wang T, Zheng T, Teng J, Chen J. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 2016;7:10151 pubmed publisher
	Herman J, Miller M, Biggins S. chTOG is a conserved mitotic error correction factor. elife. 2020;9: pubmed publisher
	Shirnekhi H, Kelley E, DeLuca J, Herman J. Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation. Mol Biol Cell. 2017;28:2035-2041 pubmed publisher

product information

Catalog No :

GTX109519

Product Name :

MAD1 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

22024768,22351768,22581055

Product Description :

Rabbit Polyclonal antibody to MAD1 (MAD1 mitotic arrest deficient-like 1 (yeast))

CrossReactivity :

Hu, Ms, Rat

Background :

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB, ChIP assay

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using Mad 1 (GTX109519) antibody at 1:100 dilution.

Image Legend_5 :

MAD1 antibody detects MAD1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody (GTX109519) diluted by 1:5000.

Image Legend_6 :

Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody (GTX109519). Western blot analysis was performed using MAD1 antibody (GTX109519). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_7 :

MAD1 antibody detects MAD1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody (GTX109519) diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] (GTX70268) diluted at 1:500. Blue: Hoechst 33342 staining.