NFkB p65 antibody | GeneTex GTX107678 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

NFkB p65 antibody

catalog :

GTX107678

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, EMSA, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:1000; fig 6e	Shang S, Ji X, Zhang L, Chen J, Li C, Shi R, et al. Macrophage ABHD5 Suppresses NFκB-Dependent Matrix Metalloproteinase Expression and Cancer Metastasis. Cancer Res. 2019;79:5513-5526 pubmed publisher

product information

Catalog No :

GTX107678

Product Name :

NFkB p65 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

20367285,21767632,21336280,24646150,24758390,25945832,25945832

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))

CrossReactivity :

Hu, Ms

Background :

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

EMSA, ICC/IF, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Image Legend_5 :

NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_6 :

NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000.

Image Legend_7 :

NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.