PGK1 antibody | GeneTex GTX107614 product information

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product summary

company name :

GeneTex

product type :

antibody

product name :

PGK1 antibody

catalog :

GTX107614

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

product information

Catalog No :

GTX107614

Product Name :

PGK1 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

24011924

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to PGK1 (phosphoglycerate kinase 1)

CrossReactivity :

Hu, Ms, Rat, Yeast

Background :

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ELISA, ICC/IF, IHC-P, WB

Concentration :

0.48mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

PGK1 antibody detects PGK1 protein by Western blot analysis. A. 50 µg Rat liver lysate/extract 10% SDS-PAGE PGK1 antibody (GTX107614) dilution: 1:1000

Image Legend_5 :

Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.

Image Legend_6 :

Sample (30 ug of whole cell lysate) A: A549 B: Hela 10% SDS PAGE GTX107614 diluted at 1:1000

Image Legend_7 :

PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.