Bcl-X antibody | GeneTex GTX105661 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Bcl-X antibody

catalog :

GTX105661

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 5	Huang Y, Chen Y, Lai Y, Cheng C, Lin T, Su Y, et al. Resveratrol alleviates the cytotoxicity induced by the radiocontrast agent, ioxitalamate, by reducing the production of reactive oxygen species in HK-2 human renal proximal tubule epithelial cells in vitro. Int J Mol Med. 2016;37:83-91 pubmed publisher

product information

Catalog No :

GTX105661

Product Name :

Bcl-X antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

20705336

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Bcl-X (BCL2-like 1)

CrossReactivity :

Hu, Ms, Rat

Background :

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human Bcl-X. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB

Concentration :

0.95mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Bcl-X antibody detects BCL2L1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12 % SDS-PAGE Bcl-X antibody (GTX105661) dilution: 1:1000

Image Legend_5 :

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1(GTX105661) antibody at 1:500 dilution.

Image Legend_6 :

Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 μg of Bcl-X antibody (GTX105661). Western blot analysis was performed using Bcl-X antibody (GTX105661). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_7 :

Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bcl-X protein stained by Bcl-X antibody (GTX105661) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.