NRF2 antibody [N2C2], Internal | GeneTex GTX103322 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

NRF2 antibody [N2C2], Internal

catalog :

GTX103322

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5b	Ast T, Meisel J, Patra S, Wang H, Grange R, Kim S, et al. Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis. Cell. 2019;: pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; tbl 2	Draheim T, Liessem A, Scheld M, Wilms F, WeiÃŸflog M, Denecke B, et al. Activation of the astrocytic Nrf2/ARE system ameliorates the formation of demyelinating lesions in a multiple sclerosis animal model. Glia. 2016;64:2219-2230 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:50; fig 4	Arellano BuendÃa A, Tostado GonzÃ¡lez M, GarcÃa Arroyo F, CristÃ³bal GarcÃa M, Loredo Mendoza M, Tapia E, et al. Anti-Inflammatory Therapy Modulates Nrf2-Keap1 in Kidney from Rats with Diabetes. Oxid Med Cell Longev. 2016;2016:4693801 pubmed publisher
	Batubara I, Astuti R, Prastya M, Ilmiawati A, Maeda M, Suzuki M, et al. The Antiaging Effect of Active Fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level. Antioxidants (Basel). 2020;9: pubmed publisher
	Choi E, Jung B, Lee S, Yoo H, Shin E, Ko H, et al. A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017;36:5285-5295 pubmed publisher

product information

Catalog No :

GTX103322

Product Name :

NRF2 antibody [N2C2], Internal

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

25571843,25571843

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to NRF2 (nuclear factor (erythroid-derived 2)-like 2)

CrossReactivity :

Hu, Rat

Background :

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Buffer :

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Uses :

ICC/IF, IHC-P, IP, WB, ChIP assay

Concentration :

0.56mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000.

Image Legend_5 :

NRF2 antibody detects NRF2 protein by western blot analysis. Un-treated (-) and treated (+, 30μM tBHQ treatment for 4h) MDA-MB-231 NE whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:500.

Image Legend_6 :

Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal (GTX103322). Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.