Oct4 antibody | GeneTex GTX101497 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Oct4 antibody

catalog :

GTX101497

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; fig 4	Lee T, Liu C, Chang Y, Nieh S, Lin Y, Jao S, et al. Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative. Oncotarget. 2016;7:23512-20 pubmed publisher
	Feng Y, Su Y, Lin S, Lin P, Wu C, Tung C, et al. Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer. BMC Cancer. 2019;19:791 pubmed publisher

product information

Catalog No :

GTX101497

Product Name :

Oct4 antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

22153870,23810380

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)

CrossReactivity :

Hu, Ms

Background :

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing s sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Buffer :

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Uses :

FACS, ICC/IF, IHC-P, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_5 :

Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX101497 diluted at 1:10000

Image Legend_6 :

Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Image Legend_7 :

Oct4 antibody detects Oct4 protein by western blot analysis. A. 30 μg human ESC whole cell extract . 12 % SDS-PAGE. Oct4 antibody (GTX101497) dilution: 1:10000

Image Legend_8 :

OCT4 antibody detects OCT4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT4 antibody (GTX101497) at a dilution of 1:50000.