LDHA antibody | GeneTex GTX101416 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

LDHA antibody

catalog :

GTX101416

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1e	Thompson J, Nguyen Q, Singh M, Pavesic M, Nesterenko I, Nelson L, et al. Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma. Oncogene. 2017;36:1080-1089 pubmed publisher

product information

Catalog No :

GTX101416

Product Name :

LDHA antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Lactate Dehydrogenase (lactate dehydrogenase A)

CrossReactivity :

Hu, Ms, Rat

Background :

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.

Buffer :

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, WB

Concentration :

0.88mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

LDHA antibody detects LDHA protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 10 % SDS-PAGE LDHA antibody (GTX101416) dilution: 1:1000

Image Legend_5 :

LDHA antibody detects LDHA protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg Raw264.7 whole cell lysate/extract E. 30 μg C2C12 whole cell lysate/extract 10 % SDS-PAGE LDHA antibody (GTX101416) dilution: 1:1000

Image Legend_6 :

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.

Image Legend_7 :

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.