PTEN antibody | GeneTex GTX101025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

PTEN antibody

catalog :

GTX101025

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Chen N, Chyau C, Lee Y, Tseng H, Chou F. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells. Sci Rep. 2016;6:20417 pubmed publisher

product information

Catalog No :

GTX101025

Product Name :

PTEN antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

21477582,25061500

Product Description :

Rabbit Polyclonal antibody to PTEN (phosphatase and tensin homolog)

CrossReactivity :

Hu, Ms, Rat

Background :

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human PTEN. The exact sequence is proprietary.

Buffer :

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ICC/IF, IHC-P, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PTEN(GTX101025) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Image Legend_5 :

Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE GTX101025 diluted at 1:1000

Image Legend_6 :

PTEN antibody detects PTEN protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PTEN antibody (GTX101025) dilution: 1:500.

Image Legend_7 :

PTEN antibody detects PTEN protein at cytosol and nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. PTEN antibody (GTX101025) dilution: 1:500.