Nanog antibody [N3C3] | GeneTex GTX100863 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

Nanog antibody [N3C3]

catalog :

GTX100863

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 1

Reference
Hildebrandt T, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree M, et al. Embryos and embryonic stem cells from the white rhinoceros. Nat Commun. 2018;9:2589 pubmed publisher

product information

Catalog No :

GTX100863

Product Name :

Nanog antibody [N3C3]

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

23288320,22641621,24607276

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to Nanog (Nanog homeobox)

CrossReactivity :

Hu, Ms

Background :

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Immunogen :

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Buffer :

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Uses :

ELISA, ICC/IF, IHC, IP, WB

Concentration :

0.5mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.

Image Legend_5 :

Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863). Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Image Legend_6 :

Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000

Image Legend_7 :

NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 6 μm.