PCNA antibody | GeneTex GTX100539 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

PCNA antibody

catalog :

GTX100539

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:500; fig 7	Liu T, Mu H, Shen Z, Song Z, Chen X, Wang Y. Autologous adipose tissueâ€‘derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy. Mol Med Rep. 2016;13:2053-9 pubmed publisher
	Garc a Arroyo F, Monroy S nchez F, Mu oz Jim nez I, Gonzaga G, Andr s Hernando A, Zazueta C, et al. Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats. Biomolecules. 2019;9: pubmed publisher
	Li R, Yi X, Wei X, Huo B, Guo X, Cheng C, et al. EZH2 inhibits autophagic cell death of aortic vascular smooth muscle cells to affect aortic dissection. Cell Death Dis. 2018;9:180 pubmed publisher

product information

Catalog No :

GTX100539

Product Name :

PCNA antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

22306340,22344225,23640890,24763901,24818710,25012905,25242635,25429617

Isotype :

IgG

Product Description :

Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)

CrossReactivity :

Hu, Ms, Rat

Background :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]

Immunogen :

Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

Buffer :

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Uses :

ICC/IF, IHC, IHC-P, WB

Concentration :

0.11mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Image Legend_4 :

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PCNA(GTX100539) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Image Legend_5 :

PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PCNA antibody (GTX100539) dilution: 1:500.

Image Legend_6 :

PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody (GTX100539) dilution: 1:500.

Image Legend_7 :

PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PCNA antibody (GTX100539) dilution: 1:500.

Image Legend_8 :

PCNA antibody detects PCNA protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) at a dilution of 1:2500.