mouse monoclonal (17F8)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 16
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 16
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
BRCA1 antibody 17F8 (GTX70111) was used for WB at 1:1000 dilution. MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate were used in the Western blot assay with a 5-15% gradient gel.
quantity: 100.00μg
price: 299 USD
to the supplier
mouse monoclonal (8F7)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 13
reactivity: human, mouse
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 13
mouse monoclonal (6B4)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 4
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 4
BRCA1 antibody 6B4 (GTX70115) was used for immunofluorescent staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used at 1:400 dilution. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4).
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
BRCA1 antibody 6B4 (GTX70115) was used for Western blot assay at 1:1000 antibody dilution. Western blot assay was performed using MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate separated in a 5-15% gradient gel. Observed molecular weight of BRCA1: 220 KD. Predicted molecular weight of BRCA1: 220 KD.
quantity: 100.00μg
price: 299 USD
to the supplier
mouse monoclonal (9F12)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRCA1(GTX70117) antibody at 1:100 dilution.
quantity: 100.00μg
price: 299 USD
to the supplier