rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunocytochemistry, immunohistochemistry - frozen section
citations: 2
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunocytochemistry, immunohistochemistry - frozen section
citations: 2
Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were probed with 0.35 µg/mL GTX24803 antibody following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-tyrosine containing peptide (3), a phosphopeptide derived from the corresponding region of Pyk2 (4), or, the phosphopeptide immunogen (5). The data show that only the peptide corresponding to GTX24803 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
Visualizing focal adhesion kinase with FAK [pY397] PSSA. HeLa cells were fixed and permeabilized using ice-cold 95% methanol. FAK [pY397] PSSA was labeled with Alexa Fluor® 488 dye (green fluorescence), and nuclei were counterstained with DAPI (blue fluorescence). The image was captured using a fluorescence microscope fitted with a 100× oil immersion lens.
FAK [pY397] PSSA (GTX24803) analysis of CEF cells illustrate that only the phosphopeptide corresponding to FAK [pY397] blocks the Western blot signal.
quantity: 50.00μl
price: 289 USD
to the supplier
rabbit polyclonal
reactivity: human
citations: 3
reactivity: human
citations: 3
rabbit polyclonal
reactivity: human
reactivity: human
rabbit polyclonal
reactivity: human
reactivity: human