rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 12
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 12
HIF1 alpha antibody immunoprecipitates HIF1 alpha protein in IP experiments. IP Sample: 950 µg whole cell lysate/extract of HIF1 alpha protein expressing HeLa cells. A. 20 ?g whole cell lysate/extract of transfected HeLa cells B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of HIF1 alpha protein by 2.5 ?g of HIF1 alpha antibody (GTX127309) 7.5% SDS-PAGE The immunoprecipitated HIF1 alpha protein was detected by HIF1 alpha antibody (GTX127309) diluted at 1:10000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis. Samples: Untreated (left panel) and 500?M CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500 Blue: Hoechst 33342 staining. Scale bar = 10 ?m. Images captured by Olympus FV10i Confocal Laser Scanning Microscope
HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated) B. 30 µg NIH-3T3 whole cell lysate/extract (200µM CoCl2 treatment for 24hr) HIF1 alpha antibody (GTX127309) dilution: 1:1000
quantity: 100.00μl
price: 299 USD
to the supplier
mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 3
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 3
Immunohistochemical staining of human globlastoma multiforme utilizing GTX30105.
Immunoprecipitation using HIF-1 alpha Antibody [H1alpha67] (GTX30105) - Heavy and light chains are also detected.
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-HIF-1 alpha antibody [H1alpha67] (GTX30105).
quantity: 100.00μl
price: 299 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [GTX30647] - Detection of HIF-1 Alpha (green) in RCC4 cells using GTX30647. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunohistochemistry: HIF-1 alpha Antibody [GTX30647] - Staining of placenta, villi.
Immunohistochemistry: HIF-1 alpha Antibody [GTX30647] - Analysis of HIF-1a in normal mouse kidney
quantity: 100.00μl
price: 299 USD
to the supplier
mouse monoclonal (GT10211)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments. IP Sample: 1000 µg HepG2 whole cell lysate/extract A. 40 ?g HepG2 whole cell lysate/extract from normoxia B. 40 ?g HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2) C. Control with 2.5 ?g of preimmune mouse IgG/HepG2 whole cell lysate/extract from normoxia D. Control with 2.5 ?g of preimmune mouse IgG/ HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2) E. Immunoprecipitation of HIF-1 alpha protein by 2.5 ?g of HIF-1 alpha antibody (GTX628480)/HepG2 whole cell lysate/extract from normoxia F. Immunoprecipitation of HIF-1 alpha protein by 2.5 ?g of HIF-1 alpha antibody (GTX628480)/HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2) 10% SDS-PAGE The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
HIF1 alpha antibody [GT10211] detects HIF1A protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr) 7.5 % SDS-PAGE HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250
HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500?M CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
quantity: 250.00μl
price: 299 USD
to the supplier