domestic rabbit polyclonal
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 8
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 8
Immunohistochemical analysis (whole mount) of transgenic zebrafish embryo, using GFP(GTX113617) antibody at 1:200 dilution.
IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.
GFP antibody detects GFP protein by Western blot analysis. A. 1 µg 293T whole cell extract B. 1 µg 293T whole cell extract expressing GFP-tagged protein C. 1 µg 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE GFP antibody (GTX113617) dilution: 1:10000
quantity: 100.00μl
price: 299 USD
to the supplier
domestic goat polyclonal
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1
These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord. This was detected using GTX26662 and a standard FITC filter set. Axons are labelled red by an antibody to neurofilamen
Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.
quantity: 100.00μg
price: 259 USD
to the supplier
mouse monoclonal (GT859)
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 7
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 7
ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection. Coating Ab: mouse anti-GFP (GTX628528, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP (GTX113617) Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)
GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.
GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 µg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ?g lysate/extract of transfected 293T cell B. Control with 2 ?g of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ?g of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
quantity: 100.00μl
price: 299 USD
to the supplier
chicken polyclonal
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3
Left Panel - GFP immunostaining of neurons in the spinal cord of a transgenic mouse expressing GFP under the control of a gene product specifically expressed in laminae 2 of the dorsal horn gray matter. Right Panel - Western blot showing a single band at about 27 kDa, the molecular weight of GFP.
Left Panel - GFP immunostaining of neurons in the spinal cord of a transgenic mouse expressing GFP under the control of a gene product specifically expressed in laminae 2 of the dorsal horn gray matter. Right Panel - Western blot showing a single band at about 27 kDa, the molecular weight of GFP.
quantity: 50.00μl
price: 259 USD
to the supplier
chicken polyclonal
application: western blot
citations: 1
application: western blot
citations: 1
