rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Tau antibody detects MAPT protein at cytosol on D54MG xenograft by immunohistochemical analysis. Sample: Paraffin-embedded D54MG xenograft. Tau antibody (GTX100866) dilution: 1:500.
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX100866 diluted at 1:3000
Tau antibody detects Tau protein by Western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract. B. 30 µg IMR32 whole cell lysate/extract. C. 30 µg SK-N-AS whole cell lysate/extract. 10 % SDS-PAGE. Tau antibody (GTX100866) dilution: 1:500
quantity: 100.00μl
price: 299 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry - paraffin section
Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer’s Disease using Tau (Phospho-Ser396) Antibody (GTX50166).
Western blot analysis of extracts from mouse brain tissue using Tau (Phospho-Ser396) Antibody (GTX50166) and the same antibody preincubated with blocking peptide.
quantity: 100.00μl
price: 299 USD
to the supplier
rabbit monoclonal (EPR2402)
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunoprecipitation
rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot
Human recombinant Tau stimulated with GSK-3beta (1 µg per µg Tau) for 45 minutes was added to background extracts, resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for 1h at room temperature and either left untreated (1-4) or treated with lambda phosphatase (5), then incubated with the Tau [pS422] antibody in a 3% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1,5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method.The data show that only the phosphopeptide corresponding to Tau [pS422] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
Tau protein schematic showing important functional domains and phosphorylation sites
quantity: 50.00μl
price: 289 USD
to the supplier