FAK (phospho Tyr397) antibody
GeneTex
catalog: GTX24803

rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: western blot, immunocytochemistry, immunohistochemistry - frozen section
citations: 2

Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were probed with 0.35 µg/mL GTX24803 antibody following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-tyrosine containing peptide (3), a phosphopeptide derived from the corresponding region of Pyk2 (4), or, the phosphopeptide immunogen (5).  The data show that only the peptide corresponding to GTX24803 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Visualizing focal adhesion kinase with FAK [pY397] PSSA. HeLa cells were fixed and permeabilized using ice-cold 95% methanol. FAK [pY397] PSSA was labeled with Alexa Fluor® 488 dye (green fluorescence), and nuclei were counterstained with DAPI (blue fluorescence). The image was captured using a fluorescence microscope fitted with a 100× oil immersion lens.

FAK [pY397] PSSA (GTX24803) analysis of CEF cells illustrate that only the phosphopeptide corresponding to FAK [pY397] blocks the Western blot signal.
quantity: 50.00μl
price: 289 USD
to the supplier

FAK (phospho Tyr397) antibody [EP2160Y]
GeneTex
catalog: GTX61795

rabbit monoclonal (EP2160Y)
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunocytochemistry

A. Western blot analysis on human brain tissue lysates (A B) and rat brain tissue lysates (C D) using anti-Phospho-Fak (pY397) RabMAb (cat. #GTX61795) 1:2000 dilution. Cells were either (A C) untreated (B D) treated with AP
quantity: 100.00μl
price: 329 USD
to the supplier

FAK (phospho Ser722) antibody
GeneTex
catalog: GTX24790

rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot

Extracts of HeLa cells treated with 100 ng/mL Taxol for 24 hours were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.
quantity: 50.00μl
price: 289 USD
to the supplier

FAK (phospho Ser910) antibody
GeneTex
catalog: GTX24794

rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot

Extracts of human epithelial carcinoma cells expressing FAK with (2-5) or without (1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF
quantity: 50.00μl
price: 299 USD
to the supplier