rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - frozen section
citations: 3
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - frozen section
citations: 3
rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
citations: 3
reactivity: human
application: western blot, immunocytochemistry
citations: 3
Sample (30 ug of whole cell lysate) A: HCT116 B: HCT116 treated with 30uM cisplatin for 24hr 12% SDS PAGE GTX100444 diluted at 1:1000
p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Samples: HCT116 cells mock and treated with 30?M cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX100444) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 ?m.
p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Samples: HCT116 cells mock (left) and treated with 30 ?M Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX100444) diluted at 1:1000. Red: Histone H3S10ph (phospho Ser10), a nucleus marker, stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:500. Blue: Hoechst 33342 staining.
quantity: 100.00μl
price: 299 USD
to the supplier
mouse monoclonal (GT1032)
reactivity: human
citations: 4
reactivity: human
citations: 4
p21 Cip1 antibody [GT1032] detects p21 Cip1 protein by Western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated) B. 30 µg HCT116 whole cell lysate/extract (30 µM Cisplatin treatment for 24 hr) C. 30 µg HCT116 whole cell lysate/extract (30 µM Cisplatin treatment for 48 hr) 15 % SDS-PAGE p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:1000
p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells treated with 30?M cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. Blue: Hoechst 33342 staining.
p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments. IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ?g HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ?g of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ?g p21 Cip1 antibody [GT1032] (GTX629543) 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. [EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
quantity:
price:
to the supplier
rabbit polyclonal
reactivity: human
citations: 4
reactivity: human
citations: 4
mouse monoclonal
reactivity: human
reactivity: human