H2AX Polyclonal Antibody | Epigentek A-7701-100 product information

product summary

company name :

Epigentek

product type :

antibody

product name :

H2AX Polyclonal Antibody

catalog :

A-7701-100

quantity :

100 µl

price :

567.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Epigentek product webpage

product information

Catalog# :

A-7701

Part # :

A-7701-100

2022 Price (US) :

567.00 USD

Product Description (Product Name) :

H2AX Polyclonal Antibody

Size :

100 µl

Host :

Rabbit

Antigen (Uniprot Accession, Gene or prot ... :

H2AX

Conjugate :

Unconjugated

Clonality :

Polyclonal

Species/Reactivity :

Human, Mouse, Rat, Monkey

Applications :

WB, IHC, ICC; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 ICC 1:50 - 1:200

Isotype :

IgG

Immunogen :

Recombinant Protein of Human H2AFX

Shipping Temp :

Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

Uniprot ID :

P16104

Gene :

H2AFX

Storage Buffer :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purity :

Affinity Purified

Alternative Names :

H2AFX, H2A.X, H2A/X, H2AX, Histone H2A.x

Background :

Histone H2A. X is a variant histone of H2A and is necessary for DNA repair and checkpoint-mediated cell cycle arrest that occurs after double-stranded DNA breaks. DNA damage from ionizing radiation, radiomimetic agents, or UV-light causes rapid phosphorylation of H2A. X at Ser139 via PI3K-like kinases. Very soon after DNA damage occurs, H2A. X is phosphorylated at Ser139 at sites of DNA damage. This is required for the recruitment of DNA-damage response proteins, such as MDC1, NBS1, RAD50, MRE11, 53BP1, and BRCA1. H2A. X is also involved in the fragmentation of DNA during apoptosis and, in response to apoptotic signals, is phosphorylated by various kinases. H2A. X can be phosphorylated at Ser139 by DNA-PK, c-Jun N-terminal Kinase (JNK1), and p38 MAPK. In undamaged cells, H2A. X is phosphorylated on Tyr142 by WSTF (Williams-Beuren syndrome transcription factor). Tyr142 is dephosphorylated at DNA damage sites by EYA1 and EYA3 phosphatases. This happens as soon as DNA damage and phosphorylation of Ser139 occur. Phosphorylation at Ser139 helps recruit DNA repair and apoptotic proteins to DNA damage sites, and phosphorylation at Tyr142 determines which set of proteins are recruited. H2A. X phosphorylation at Tyr142 inhibits the enlistment of DNA repair proteins and endorses binding of pro-apoptotic factors such as JNK1. Fibroblasts studied in mouse embryos that only express mutant H2A. X Y142F display diminished apoptotic response to ionizing radiation. This suggests that the balance of H2A. X Tyr142 phosphorylation and dephosphorylation acts as a switch mechanism in determining cell fate following DNA damage.

more info or order :

Epigentek product webpage