Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody | Epigentek A-4031-100 product information

product summary

company name :

Epigentek

product type :

antibody

product name :

Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody

catalog :

A-4031-100

quantity :

100 µl

price :

411.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

more info or order :

Epigentek product webpage

product information

Catalog# :

A-4031

Part # :

A-4031-100

2022 Price (US) :

411.00 USD

Product Description (Product Name) :

Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody

Size :

100 µl

Host :

Rabbit

Antigen (Uniprot Accession, Gene or prot ... :

H3K4me1

Conjugate :

Unconjugated

Clonality :

Polyclonal

Species/Reactivity :

Broad Range, Mouse, Rat, Human

Applications :

WB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB: 1:500-1:2000, IHC: 1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP: 1:20-1:100, ChIP-seq: 1:20-1:100

Isotype :

IgG

Immunogen :

A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Shipping Temp :

Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Uniprot ID :

Q16695

Gene :

H3K4me1

Storage Buffer :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purity :

Affinity Purification

Alternative Names :

H3K4me1 antibody; H3K4m1 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3

Background :

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

more info or order :

Epigentek product webpage