antibody

CBX5 Polyclonal Antibody

A-2701-100

100 µl

308.00 USD

polyclonal

domestic rabbit

nonconjugated

polyclonal

human, rat

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

A-2701-100

308.00 USD

CBX5 Polyclonal Antibody

100 µl

Rabbit

CBX5

Unconjugated

Polyclonal

Human, Rat

WB, IF, IP, ChIP; Recommended dilution: WB: 1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP: 1:20-1:100

IgG

Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CBX5 (NP_001120794.1).

Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

P45973

CBX5

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Affinity Purification

CBX5, HP1, HP1A

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (alpha, beta, and gamma) are primarily associated with centromeric heterochromatin; however, HP1beta and gamma also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.