antibody

SARS-CoV-2 Furin Site Blocking Polyclonal Antibody

A-1810-100

100 μl

369.00 USD

polyclonal

domestic rabbit

nonconjugated

polyclonal

ELISA, immunoprecipitation

A-1810-100

369.00 USD

SARS-CoV-2 Furin Site Blocking Polyclonal Antibody

100 μl

Rabbit

SARS-CoV-2 Spike

Unconjugated

Polyclonal

SARS-CoV-2

ELISA, IP, SARS-CoV-2-specific furin cleavage site blockage; Recommended dilution: ELISA: 1:500-1:5000; IP: 2-10 µg (per reaction)

IgG

https://www.biorxiv.org/content/10.1101/2021.02.09.430451v1

Peptide-KLH conjugate consisting of the SARS-CoV-2-specific furin motif (20 aa)

Shipped at 4°C. Upon receipt, store at 4°C for short term (3-4 weeks) and -20°C for long term (18 months). Avoid repeated freeze.

P0DTC2

Spike glycoprotein

Liquid. 50% glycerol, PBS, pH 7.4

Affinity Purified

fbAB

SARS-CoV-2 harbors a unique furin cleavage site at the boundary between the S1 and S2 subunits of its spike protein, which can be cleaved by the proprotein convertase (PC) furin and furin-like PCs. Proteolytic activation of SARS-CoV-2 spike protein at the S1/S2 boundary facilitates interaction with host ACE2 receptor for cell entry. The complete SARS-CoV-2 furin cleavage site has been characterized as a 20 amino acid motif corresponding to the amino acid sequence A672- S691 of SARS-CoV-2 spike protein, with one core region SPRRAR│SV (8 amino acids, S680- V687) and two flanking solvent-accessible regions (8 amino acids, A672– N679, and 4 amino acids, A688- S691). The core region is very unique as its R683 and A684 positions are positively-charged (Arg) and hydrophobic (Ala) residues, respectively, which could be cleaved by furin and/or furin-like PCs secreted from host cells and bacteria in the airway epithelium. Furin and furin-like PCs, such as PC5/6A and PACE4, are proven to be cleavage region sequence-specific, and these PCs exhibit widespread tissue distribution.