antibody

N6-methyladenosine (m6A) Monoclonal Antibody [2H6]

A-1802-100

100 µl

380.00 USD

monoclonal

domestic rabbit

nonconjugated

monoclonal

human, mouse, rat

ELISA, immunocytochemistry

A-1802-100

380.00 USD

N6-methyladenosine (m6A) Monoclonal Antibody [2H6]

100 µl

Rabbit

m6A

Unconjugated

Monoclonal

Human, Mouse, Rat, Broad Range

ELISA, DB, IF, MeRIP, Nucleotide Array; Recommended dilution: DB: 1:500 - 1:2000, IF: 1:50 - 1:200

IgG

Chemical compounds corresponding to N6-methyladenosine / m6A

Shipped at 4°C. Upon delivery store at 20°C or -80°C (long-term). Avoid repeated freeze.

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Affinity Purified

Anti-N6-methyladenosine, N6-methyladenosine, N6(methylnitroso)adenosine, m6A, N6-methyladenosine, 6mA

N6-methyladenosine (m6A) is the most common and abundant modification in RNA molecules present in eukaryotes. The m6A modification is catalyzed by a methyltransferase complex METTL3 and removed by the recently discovered m6A RNA demethylases FTO and ALKBH5, which catalyze m6A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. It was shown that METTL3, FTO, and ALKBH5 play essential roles in many biological processes, ranging from development and metabolism to fertility. m6A accounts for more than 80% of all RNA base methylations and exists in various species. m6A is mainly distributed in mRNA and also occurs in non-coding RNA such as tRNA, rRNA, and snRNA. The relative abundance of m6A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. Abnormal m6A methylation levels induced by defects in m6A RNA methylase and demethylase could lead to dysfunction of RNA and diseases. For example, abnormally low levels of m6A in target mRNAs due to increased FTO activity in patients with FTO mutations, through an as-yet-undefined pathway, contributes to the onset of obesity and related diseases. The dynamic and reversible chemical m6A modification in RNA may also serve as a novel epigenetic marker of profound biological significance. Therefore, more useful information for a better understanding of m6A RNA methylation levels and distribution on RNA transcripts could benefit diagnostics and therapeutics of disease.