DNMT3L Polyclonal Antibody | Epigentek A-1005-100 product information

product summary

company name :

Epigentek

product type :

antibody

product name :

DNMT3L Polyclonal Antibody

catalog :

A-1005-100

quantity :

100 µl

price :

403.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Epigentek product webpage

product information

Catalog# :

A-1005

Part # :

A-1005-100

2022 Price (US) :

403.00 USD

Product Description (Product Name) :

DNMT3L Polyclonal Antibody

Size :

100 µl

Host :

Rabbit

Antigen (Uniprot Accession, Gene or prot ... :

DNMT3L

Conjugate :

Unconjugated

Clonality :

Polyclonal

Species/Reactivity :

Human, Mouse, Rat

Applications :

WB, IHC, IF; Recommended dilution: WB: 1:500-1:2000, IHC: 1:50-1:200, IF:1:20-1:50

Isotype :

IgG

Citations :

33298433

Immunogen :

Recombinant fusion protein containing a sequence corresponding to amino acids 1-386 of human DNMT3L (NP_787063.1).

Shipping Temp :

Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Uniprot ID :

Q9UJW3

Gene :

DNMT3L

Storage Buffer :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purity :

Affinity Purification

Alternative Names :

DNA Cytosine 5 Methyltransferase 3 Like Protein antibody, DNA Methyltransferase 3 Like Protein antibody, DNM3L_HUMAN antibody, Dnmt 3L antibody, Human cytosine 5 methyltransferase 3 like protein antibody, MGC1090 antibody

Background :

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development . Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain. The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA. The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4.

more info or order :

Epigentek product webpage