Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

ELISA/assay

VEGF (human), ELISA kit

ENZ-KIT156-0001

96 wells

ENZ-KIT156-0001

VEGF (human), ELISA kit

Kit

96 wells

The VEGF (human), EIA kit is a sensitive colorimetric immunometric enzyme immunoassay kit with results in just 2.5 hours. Absorbance is read at 450 nm. The wide dynamic range is ideal for various matrices. Assay up to 39 samples in duplicate with each kit.

Human

Vascular Endothelial Growth Factor (VEGF), originally known as vascular permeability factor (VPF), is a family of proteins that stimulate vasculogenesis and angiogenesis. The VEGF family comprises five members: VEGF-A, VEGF-B, VEGF-C, VEGF-D and PGF (placenta growth factor). Being the first VEGF family member discovered, VEGF-A is often simply referred to as VEGF. Alternative splicing of an 8-exon VEGF mRNA results in multiple isoforms of 121, 145, 165, 183, 189, and 206 amino acids. VEGF165 and VEGF121 are predominant isoforms expressed in most cells and tissues. VEGF165 is a 45 KDa homodimeric glycoprotein and has moderate affinity to heparin.  In contrast, VEGF 121 doesn’t bind heparin. VEGF specifically binds tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (Flk-1) to activate downstream signaling pathways leading to endothelial cell survival, proliferation, migration, and vascular permeability. VEGF plays an essential role in normal vascular embryonic and reproductive angiogenesis. On the other hand, VEGF is central to pathological angiogenesis by enhancing vessel permeability or recruiting endothelial progenitor cells to distant sites of neovascularization. The well understanding of VEGF in promoting pathological angiogenesis and tumor metastasis has led to the rational studies on blocking the VEGF/VEGFR axis in inhibiting angiogenesis and tumor growth in multiple clinical models.

Vascular endothelial growth factor

For the quantitative determination of human VEGF in serum, plasma, and culture supernatants.

ELISA, Colorimetric detection

+4°C