Stressgen, BioMol, Alexis Corporation, Assay Designs, Alexis

ELISA/assay

Melatonin ELISA Kit

ENZ-KIT150-0001

96 wells

ENZ-KIT150-0001

Melatonin ELISA Kit

Kit

96 wells

The Melatonin ELISA kit is a colorimetric competitive immunoassay kit with results in 2 hours.

Species independent

Melatonin (N-acetyl-5-methoxytryptamine) is produced in the pineal gland and is a major component of the regulation of the circadian rhythm. Melatonin is produced from tryptophan through a number of hydroxylation and methylation steps. It is metabolized in the liver, and excreted in urine as 6-hydroxymelatonin and 6-sulfatoxymelatonin. Melatonin and metabolites of melatonin, have demonstrated antioxidant and free radical scavenging potential. There is also evidence that they play a role in the regulation of reactive oxygen protein pathways. Published research shows a possible correlation to melatonin levels and the prevalence of certain cancers. Saliva can be used to measure melatonin levels. Saliva, compared to serum or plasma, is relatively easy to obtain and does not contain many of the interfering substances present in serum and plasma. Melatonin in saliva is representative of the levels circulating throughout the body, although the exact proportion is still not fully quantified with published values ranging from 20-50 % of serum values. New areas of research have grown around studying melatonin and the effect on libido, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and other hormone pathways. The role in cancer, sleep cycle, aging, and other areas are also growing in interest, with particular focus on melatonin receptors and the bioavailability of circulating melatonin with the increased use of melatonin as a dietary supplement.

N-acetyl-5-methoxytryptamine

For the quantitative determination of melatonin in any species. Validated specifically for saliva, serum, plasma, and fruit samples.

ELISA, Colorimetric detection

-20°C